05-516
Anti-KRAS Antibody
UPSTATE®, mouse monoclonal, RAS10
Synonym(s):
GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 μg | Available to ship TODAYfromAreál Kühne+Nagel spol. s r.o. | CZK 13,200.00 |
About This Item
CZK 13,200.00
Available to ship TODAYDetails
Product Name
Anti-Ras Antibody, clone RAS10, clone RAS10, Upstate®, from mouse
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RAS10, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HRAS(3265), KRAS(3845), NRAS(4893)
General description
Immunogen
Application
1-4 μg of this antibody has been reported to immunoprecipitate Ras.
Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.
Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.
Biochem/physiol Actions
Physical form
Analysis Note
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| clone RAS10, monoclonal | clone monoclonal | clone MC57, monoclonal | clone RAS 10, monoclonal |
| antibody form purified immunoglobulin | antibody form purified antibody | antibody form culture supernatant | antibody form purified immunoglobulin |
| species reactivity rat, human, mouse | species reactivity mouse, human, rat | species reactivity rat, mouse, human | species reactivity human |
| biological source mouse | biological source mouse | biological source rabbit | biological source mouse |
| Gene Information human ... HRAS(3265), KRAS(3845), NRAS(4893) | Gene Information human ... HRAS(3265), KRAS(3845), NRAS(4893) | Gene Information human ... HRAS(3265) | Gene Information human ... KRAS(3845) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
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