05-443

Anti-VEGF Antibody, clone JH

clone JH, Upstate^®, from mouse

• Synonym(s): Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 400
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: JH, monoclonal
• species reactivity: rat, mouse, rabbit, human
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable (paraffin)
• isotype: IgG1κ
• suitability: not suitable for Western blot
• NCBI accession no.: NM_003376
• UniProt accession no.: P15692
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... VEGFA(7422)

Description

General description

34-54 kDa isoforms

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Immunogen

Recombinant human VEGF121. Clone JH121

Application

Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.

Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.

Biochem/physiol Actions

Other species not tested.

Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).

Physical form

Format: Purified

Protein G Chromatography

Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.

Preparation Note

Stable for 1 year at from date of receipt.

Analysis Note

Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.

Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable