05-419

Anti-c-Myc Tag Antibody

UPSTATE^®, mouse monoclonal, 9E10

• Synonym(s): Anti-CMyc
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43
• Clone: 9E10, monoclonal
• Technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• Application: ICC, IHC, IP, WB
• Citations: 223

Properties

• Product Name: Anti-Myc Tag Antibody, clone 9E10, clone 9E10, Upstate^®, from mouse
• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_002467.3
• UniProt accession no.: P01106
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Predicted MW for human Myc: 49 kDa

Immunogen

Synthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNS).

Application

Anti-Myc Tag Antibody, clone 9E10 is an antibody against Myc Tag for use in IH(P), IC, IP & WB.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Biochem/physiol Actions

Recognizes Myc and recombinant proteins containing the Myc epitope tag.

Physical form

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

routinely evaluated by immunoblot on a Myc-tagged recombinant protein

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable