05-253

Anti-ZAP-70 Antibody, clone 2F3.2

clone 2F3.2, Upstate^®, from mouse

• Synonym(s): Anti-ADMIO2, Anti-SRK, Anti-STCD, Anti-STD, Anti-TZK, Anti-ZAP-70
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2F3.2, monoclonal
• Application: FACS, IHC, IP, WB
• Citations: 53

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2F3.2, monoclonal
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_001079
• UniProt accession no.: P43403
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ZAP70(7535)

Description

General description

70 kDa

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.

Immunogen

GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254

Application

For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf

Research Category
Signaling

Research Sub Category
Immunological Signaling

This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.

Biochem/physiol Actions

Recognizes human ZAP-70, does not recognize murine ZAP-70.

Physical form

Format: Purified

Protein G Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable