04-820

Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal

culture supernatant, clone 60C, from rabbit

• Synonym(s): Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, estrogen receptor 1, estrogen receptor alpha, oestrogen receptor, steroid hormone receptor
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 60C, monoclonal
• Application: IP, WB
• Citations: 39

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 60C, monoclonal
• species reactivity: rat, pig, mouse, human, bovine
• packaging: antibody small pack of 25 μL
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_000116.2
• UniProt accession no.: P03372
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: bovine ... Esr1(407238) ; human ... ESR1(2099) ; mouse ... Esr1(13982) ; pig ... Esr1(397435) ; rat ... Esr1(24890)

Description

General description

66 kDa

The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.

Immunogen

Epitope: N-term

KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.

Application

Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.

Immunoprecipitation:
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Biochem/physiol Actions

Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.

Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.

Physical form

Cultured supernantant with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Routinely evaluated by western blot analysis.

Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).

Other Notes

Replaces: 05-820

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1