04-035

Anti-PTEN Antibody, clone 6H2.1

clone 6H2.1, Upstate^®, from mouse

• Synonym(s): Anti-PTEN antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6H2.1, monoclonal
• Application: IHC, IP, WB
• Citations: 27

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6H2.1, monoclonal
• species reactivity: rat, human, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_000305.3
• UniProt accession no.: O08586, P60484
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PTEN(5728), TEP1(7011)

Description

General description

55 kDa

The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.

Immunogen

C-terminal 100 amino acids of PTEN

Epitope: C-terminal region

Application

Anti-PTEN Antibody, clone 6H2.1 detects level of PTEN & has been published & validated for use in WB, IP, IH(P).

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

recognizes PTEN

Physical form

Format: Purified

Protein G Purified

Purified mouse IgG in storage buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

routinely evaluated on HeLa cell lysate

Other Notes

Replaces: 07-016

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1