Přejít k obsahu
Merck
  • Simple and accurate quantitative analysis of 20 anti-tuberculosis drugs in human plasma using liquid chromatography-electrospray ionization-tandem mass spectrometry.

Simple and accurate quantitative analysis of 20 anti-tuberculosis drugs in human plasma using liquid chromatography-electrospray ionization-tandem mass spectrometry.

Journal of pharmaceutical and biomedical analysis (2014-09-15)
Hyo-Ji Kim, Kyung-Ah Seo, Hyun-Mi Kim, Eun-Sook Jeong, Jong Lyul Ghim, Seung Heon Lee, Young Min Lee, Dong Hyun Kim, Jae-Gook Shin
ANOTACE

A simple and accurate liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method for the quantitation of 20 anti-tuberculosis (anti-TB) drugs in human plasma, was developed as a tool for therapeutic drug monitoring. Two protein precipitation methods were adopted; one using methanol containing 0.13N HCl, for precipitation of amikacin, kanamycin, streptomycin and pyrazinamide, and the other using acetonitrile, for precipitation of preamoxicillin, ciprofloxacin, clarithromycin, clofazimine, cycloserine, ethambutol, ethionamide, isoniazid, levofloxacin, linezolid, moxifloxacin, p-aminosalicylic acid (PAS), prothionamide, rifabutin, rifampin and roxithromycin. Separation was performed either on an HILIC silica column or a reversed-phase dC18 column, with a gradient elution. Detection was carried out in multiple reaction-monitoring (MRM) mode. The calibration curves were linear over a 50-fold concentration range, with correlation coefficients (r) greater than 0.9969 for all anti-TB drugs. The intra- and inter-day precision was less than 14.3%, and the accuracy ranged between 84.8 and 113.0%. The developed method was successfully applied to the identification and quantitation of anti-TB drugs in patients with multi-drug resistant TB.

MATERIÁLY
Číslo produktu
Značka
Popis produktu

Sigma-Aldrich
Clarithromycin, ≥95% (HPLC)
Supelco
Clarithromycin, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Rifabutin, >98% (HPLC), powder
USP
Levofloxacin, United States Pharmacopeia (USP) Reference Standard
Roxithromycin, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Clarithromycin, 96.0-102.0% (HPLC)
Moxifloxacin hydrochloride, European Pharmacopoeia (EP) Reference Standard
Pyrazinamide, European Pharmacopoeia (EP) Reference Standard
Clarithromycin for peak identification, European Pharmacopoeia (EP) Reference Standard
Clarithromycin, European Pharmacopoeia (EP) Reference Standard
USP
Clarithromycin, United States Pharmacopeia (USP) Reference Standard
Supelco
Moxifloxacin hydrochloride, VETRANAL®, analytical standard
USP
Amoxicillin, United States Pharmacopeia (USP) Reference Standard
Supelco
Methanol, analytical standard
Sigma-Aldrich
Pyrazinecarboxamide
Sigma-Aldrich
Rifampicin, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals
Sigma-Aldrich
Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture
Sigma-Aldrich
Ethionamide
Sigma-Aldrich
Streptomycin sulfate salt, powder
Sigma-Aldrich
Rifampicin, ≥95% (HPLC), powder or crystals
Sigma-Aldrich
Ethambutol dihydrochloride, antimycobacterial
Sigma-Aldrich
Streptomycin sulfate salt, powder, BioReagent, suitable for cell culture
Ethambutol hydrochloride, European Pharmacopoeia (EP) Reference Standard
Ethionamide, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Sigma-Aldrich
Methanol, NMR reference standard
USP
Rifabutin, United States Pharmacopeia (USP) Reference Standard
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, Absolute - Acetone free