Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Journal of nanoscience and nanotechnology (2014-04-18)

Ying Sun, Liubin Qin, Dejun Liu, Changhong Liu, Yongwei Sun, Yourong Duan

ANOTACE

Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.

MATERIÁLY

Číslo produktu

Značka

Popis produktu

265829

Sigma-Aldrich

Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

265772

Sigma-Aldrich

Gold, powder, <45 μm, 99.99% trace metals basis

349283

Sigma-Aldrich

Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

326542

Sigma-Aldrich

Gold, beads, 1-6 mm, 99.999% trace metals basis

326585

Sigma-Aldrich

Gold, powder, <10 μm, ≥99.9% trace metals basis

326526

Sigma-Aldrich

Gold, wire, diam. 0.127 mm, 99.99% trace metals basis

349305

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.997% trace metals basis

326534

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

310980

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

349275

Sigma-Aldrich

Gold, foil, thickness 0.05 mm, 99.99% trace metals basis

373168

Sigma-Aldrich

Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

265837

Sigma-Aldrich

Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis

GF92960286

Gold, tube, 50mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF23782350

Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm², permanent mylar 3.5μm support, 99.99+%

GF18334436

Gold, insulated wire, 1m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF50454132

Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF38598980

Gold, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm², permanent mylar 3.5μm support, 99.99+%

GF98812097

Gold, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

GF26034320

Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF37996789

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF33293729

Gold, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF47078129

Gold, microleaf disks, 10mm, thinness 0.5μm, specific density 966μg/cm², removable support, 99.99+%

GF43173930

Gold, rod, 25mm, diameter 3.0mm, as drawn, 99.95%

GF73794741

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF54796304

Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%

GF05586969

Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%

GF75346678

Gold, insulated wire, 0.5m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF72963221

Gold, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%

GF25294620

Gold, microfoil, disks, 10mm, thinness 0.01μm, specific density 20.7μg/cm², permanent mylar 3.5μm support, 99.99+%