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Merck

Production of the monoclonal antibody against Sudan 2 for immunoassay of Sudan dyes in egg.

Analytical biochemistry (2012-02-14)
Jing Liu, Huicai Zhang, Dongsheng Zhang, Feng Gao, Jianping Wang
ANOTACE

Many methods have been reported to determine the residues of Sudan dyes in food samples. Among the reported methods, enzyme-linked immunosorbent assay (ELISA) was a frequently used practical screen tool. In this study, a novel hapten of Sudan 2 was synthesized by coupling 4-amino-3-methylbenzoic acid to β-naphthol, and the monoclonal antibody against Sudan 2 was produced. The obtained antibody can recognize Sudan 1, 2, 3, and 4, Sudan red G, and Para red simultaneously. After evaluation of different coating antigens, a heterologous indirect competitive ELISA was then developed to determine the six Sudan dyes in egg. The cross-reactivities for the six analytes were in a range of 63% to 100%, and the limits of detection were in a range of 0.2 to 0.5 ng/g, depending on the compound. Intra- and interassay recoveries from the standard fortified blank eggs were in a range of 71.7% to 97.6% with coefficients of variation lower than 17.1%.

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Sigma-Aldrich
Sudan II, Dye content 90 %
Supelco
Sudan II, analytical standard