Přejít k obsahu
Merck

Paired immunoglobulin-like receptor B is an entry receptor for mammalian orthoreovirus.

Nature communications (2023-05-06)
Pengcheng Shang, Joshua D Simpson, Gwen M Taylor, Danica M Sutherland, Olivia L Welsh, Pavithra Aravamudhan, Rita Dos Santos Natividade, Kristina Schwab, Joshua J Michel, Amanda C Poholek, Yijen Wu, Dhivyaa Rajasundaram, Melanie Koehler, David Alsteens, Terence S Dermody
ANOTACE

Mammalian orthoreovirus (reovirus) infects most mammals and is associated with celiac disease in humans. In mice, reovirus infects the intestine and disseminates systemically to cause serotype-specific patterns of disease in the brain. To identify receptors conferring reovirus serotype-dependent neuropathogenesis, we conducted a genome-wide CRISPRa screen and identified paired immunoglobulin-like receptor B (PirB) as a receptor candidate. Ectopic expression of PirB allowed reovirus binding and infection. PirB extracelluar D3D4 region is required for reovirus attachment and infectivity. Reovirus binds to PirB with nM affinity as determined by single molecule force spectroscopy. Efficient reovirus endocytosis requires PirB signaling motifs. In inoculated mice, PirB is required for maximal replication in the brain and full neuropathogenicity of neurotropic serotype 3 (T3) reovirus. In primary cortical neurons, PirB expression contributes to T3 reovirus infectivity. Thus, PirB is an entry receptor for reovirus and contributes to T3 reovirus replication and pathogenesis in the murine brain.

MATERIÁLY
Číslo produktu
Značka
Popis produktu

Sigma-Aldrich
Sodium orthovanadate, 99.98% trace metals basis
Sigma-Aldrich
SHP1/2 PTPase Inhibitor, NSC-87877, The SHP1/2 PTPase Inhibitor, NSC-87877, also referenced under CAS 56932-43-5, controls the biological activity of SHP1/2 PTPase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
Sigma-Aldrich
Anti-GAPDH Mouse mAb (6C5), liquid, clone 6C5, Calbiochem®