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  • How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique.

How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique.

Methods in molecular biology (Clifton, N.J.) (2018-08-29)
Emmanuel Tadjuidje, Sang-Wook Cha
ANOTACE

We have taken advantage of the well-established oocyte host transfer technique to optimize a method for CRISPR editing of Xenopus that provides an efficient non-mosaic targeted insertion of small DNA fragment through homology-directed repair mechanism.

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Roche
Proteinase K, recombinant, PCR Grade, Lyophilizate from Pichia pastoris