The Malaria Metabolite HMBPP Does Not Trigger Erythrocyte Terpene Release.

Infection with malarial parasites renders hosts more mosquito-attractive than their uninfected, healthy counterparts. One volatile organic compound, α-pinene, is associated with Plasmodium spp. infection in multiple studies and is a known mosquito attractant. However, how malarial infection results in elevated levels of host-associated α-pinene remains unclear. One study suggested that exposure of erythrocytes to the malarial metabolite (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) results in increased levels of α-pinene. Here we establish that endogenous levels of α-pinene are present in human erythrocytes, that these levels vary widely by erythrocyte donor, and that α-pinene levels are not altered by HMBPP treatment.