P3243

Phosphodiesterase I from Crotalus adamanteus venom

vial of ≥100 units, Purified

• Synonyma: 5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase
• Číslo CAS: 9025-82-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-806-5
• MDL number: MFCD00131883
• Číslo EC: 3.1.4.1 (BRENDA, IUBMB)
• Specific activity: ≥20.0 unit/mg solid
• Biological source: Crotalus adamanteus venom

Vlastnosti

• biological source: Crotalus adamanteus venom
• Quality Segment: 200
• form: solid
• quality: Purified
• specific activity: ≥20.0 unit/mg solid
• packaging: vial of ≥100 units
• storage temp.: −20°C

Popis

Application

Phosphodiesterase (PDE) is any enzyme that is used to breaks phosphodiester bonds. It is a membrane-bound glycoprotein that is used to catalyze the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is used in phosphodiesterase activation assays to hydrolyze AMP. Product P3243 is from Crotalus adamanteus venom and is purified. Product P3243 has been used to hydrolyze tRNA with wyosine derivatives into mononucleosides.

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C.

Preparation Note

For testing purposes, PDE I is reconstituted in cold deionized water at 0.1 - 0,2 un/mL.

Purified via the method of Williams, et al. and further treated to inactivate contaminating 5′-nucleotidase activity.

Other Notes

One Unit hydrolyzes one μmole of p-nitrophenyl thymidine-5-phosphate per minute at 25 °C, pH 8.9

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Skladovací třída: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable