D4889
Deoxyribonucleic acid sodium salt from Escherichia coli strain B
Genomic, unsheared
Synonyma:
DNA
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Číslo CAS:
EC Number:
MDL number:
UNSPSC Code:
41106310
eCl@ss:
32160414
NACRES:
NA.52
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General description
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
Specificity
50% GC; Tm = 90.5 °C
Application
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
- PCR reactions of genomic DNA of Gardnerella vaginalis
- nick translation and radiolabeling of dNTP
- generating standard curve for 16S rDNA quantification
- transfecting neutrophils
- testing the extraction methods
Unit Definition
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
Preparation Note
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Analysis Note
Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
related product
Č. produktu
Popis
Stanovení ceny
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Zákazníci si také prohlíželi
Genomic comparisons among Escherichia coli strains B, K-12, and O157: H7 using IS elements as molecular markers
Schneider D, et al.
BMC Microbiology, 2(1), 18-18 (2002)
Johan Sukweenadhi et al.
Antonie van Leeuwenhoek, 106(5), 935-945 (2014-08-30)
Strain DCY84(T), a Gram-stain positive, rod-shaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from Yongin forest in Gyeonggi province, South Korea. Strain DCY84(T) shared the highest sequence similarity with Paenibacillus barengoltzii KACC 15270(T)
Rudolph Spangler et al.
PloS one, 4(9), e7010-e7010 (2009-09-16)
PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most
Mohamed El-Agamy Farh et al.
Journal of microbiology (Seoul, Korea), 55(10), 767-774 (2017-09-29)
Gram-staining-negative, uniflagellated, rod-shaped, designated as DCY110
Xuewen Pan et al.
Methods (San Diego, Calif.), 41(2), 206-221 (2006-12-27)
Analysis of genetic interactions has been extensively exploited to study gene functions and to dissect pathway structures. One such genetic interaction is synthetic lethality, in which the combination of two non-lethal mutations leads to loss of organism viability. We have
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