D0550

3,5-Dinitrosalicylic acid

used in colorimetric determination of reducing sugars

• Synonyma: 3,5-Dinitro-2-hydroxybenzoic acid, DNS
• Lineární vzorec: (O₂N)₂C₆H₂-2-(OH)CO₂H
• Číslo CAS: 609-99-4
• Molekulová hmotnost: 228.12
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24893243
• EC Number: 210-204-3
• Beilstein/REAXYS Number: 2220661
• MDL number: MFCD00007104
• Assay: ≥98% (HPLC and titration)
• Form: powder

Vlastnosti

• Quality Segment: 200
• assay: ≥98% (HPLC and titration)
• form: powder
• mp: 168-172 °C (lit.)
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
• InChI key: LWFUFLREGJMOIZ-UHFFFAOYSA-N

Popis

Application

3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.

Biochem/physiol Actions

3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.

Bezpečnostní informace

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302,H318
• pcodes: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Skladovací třída: 4.1A - Other explosive hazardous materials
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves