93443

Triton^™ X-100 solution

BioUltra, Molecular Biology, ~10% in H₂O

• Synonyma: Triton^™ X-100, 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
• Lineární vzorec: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
• Číslo CAS: 9036-19-5
• UNSPSC Code: 12161900
• NACRES: NA.25
• PubChem Substance ID: 57653560
• MDL number: MFCD00128254
• Beilstein/REAXYS Number: 2315025

Vlastnosti

• grade: Molecular Biology
• Quality Segment: 200
• description: non-ionic
• product line: BioUltra
• form: liquid
• mol wt: micellar avg mol wt 80,000, average mol wt 625
• concentration: ~10% in H₂O
• aggregation number: 100-155
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• refractive index: n20/D 1.349±0.002
• pH: 6.5-8.5 (25 °C)
• CMC: 0.2-0.9 mM (20-25°C), 0.23 mM
• mp: 6 °C
• transition temp: cloud point 65 °C, pour point ~7 °C
• solubility: water: soluble
• density: 1.01 g/mL at 20 °C, 1.070 g/cm³
• SMILES string: CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
• cation traces: Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤100 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
• absorption: cut-off at 310 nm
• InChI: 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
• InChI key: HNLXNOZHXNSSPN-UHFFFAOYSA-N

Popis

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton^™ X-100 has been used:

• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
• As a component of lysis buffer used for homogenization of cells
• As a component of blocking buffer in immunofluorescence staining
• For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and Benefits

• Suitable for Molecular Biology and Biochemical Research
• Free from DNase, RNase, NICKase and protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• High purity product for research applications
• Tested for the presence of Anionic and Cationic traces

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Bezpečnostní informace

• pictograms: GHS05,GHS09
• signalword: Danger
• Skladovací třída: 10 - Combustible liquids
• flash_point_f: 483.8 °F - closed cup
• flash_point_c: 251 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
• hcodes: H315,H318,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2