Přejít k obsahu
Merck
Všechny fotografie(1)

Key Documents

View All Documentation

83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Synonyma:

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
Všechny fotografie(1)

About This Item

Lineární vzorec:
C21H17ClN2O3
Číslo CAS:
62669-70-9
Molekulová hmotnost:
380.82
Beilstein/REAXYS Number:
6030951
EC Number:
263-687-8
MDL number:
MFCD00012664
UNSPSC Code:
12352116
PubChem Substance ID:
57652966
NACRES:
NA.32

grade

for fluorescence

product line

BioReagent

assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

Hledáte podobné produkty? Navštivte Průvodce porovnáváním produktů

General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
  • to label the cells for estimating the P-gp expression by flow cytometry
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
  • the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205
Wu D, et al.
Archives of Biochemistry and Biophysics, 604, 74-85 (2016)
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
Stebbins MJ, et al.
Methods, 101, 93-102 (2016)
L Benel et al.
Basic and applied histochemistry, 33(2), 71-80 (1989-01-01)
The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
Evidence of apoptosis in Raillietina echinobothrida induced by methanolic extracts of three traditional medicinal plants of Northeast India
Giri BR, et al.
Experimental Parasitology, 134(4), 466-473 (2013)
Fumihiko Takeuchi et al.
PloS one, 10(7), e0132030-e0132030 (2015-07-07)
The Myxozoa are oligo-cellular parasites with alternate hosts--fish and annelid worms--and some myxozoan species harm farmed fish. The phylum Myxozoa, comprising 2,100 species, was difficult to position in the tree of life, due to its fast evolutionary rate. Recent phylogenomic
Zobrazit všechny související dokumenty

Sortimentní položky

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.