77607

Phenol

BioUltra, Molecular Biology, TE-saturated, ~73% (T)

• Synonyma: Hydroxybenzene
• Lineární vzorec: C₆H₅OH
• Číslo CAS: 108-95-2
• Molekulová hmotnost: 94.11
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 57652703
• EC Number: 203-632-7
• Beilstein/REAXYS Number: 969616
• MDL number: MFCD00002143
• Assay: ~73% (T)
• Grade: Molecular Biology
• Bp: 182 °C (lit.)
• Vapor pressure: 0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)

Vlastnosti

• grade: Molecular Biology
• Quality Segment: 100
• vapor density: 3.24 (vs air)
• vapor pressure: 0.09 psi ( 55 °C), 0.36 mmHg ( 20 °C)
• product line: BioUltra
• assay: ~73% (T)
• form: liquid
• autoignition temp.: 1319 °F
• quality: TE-saturated
• contains: ~0.1% 8-hydroxyquinoline as preservative
• expl. lim.: 8.6 %
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• refractive index: n20/D 1.48
• pH: ~7.8(aqueous phase)
• bp: 182 °C (lit.)
• mp: 40-42 °C (lit.)
• density: 1.071 g/mL at 25 °C (lit.)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• SMILES string: Oc1ccccc1
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• suitability: suitable for molecular biology
• storage temp.: 2-8°C
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• Gene Information: human ... GABRA1(2554)

Popis

General description

saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA

Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically. It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning.

Application

Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation. It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis.

Other Notes

RNA fingerprinting - preparation of the E. coli tRNA carrier

Disclaimer

according to temperature, a small quantity of an overlaid aqueous phase may form

Bezpečnostní informace

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H314,H341,H373,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• Skladovací třída: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 2
• flash_point_f: 174.2 °F - closed cup
• flash_point_c: 79 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter