42364

1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate

BioReagent, suitable for fluorescence, ≥98.0% (TLC)

• Synonyma: DiI
• Empirický vzorec (Hillův zápis): C₅₉H₉₇ClN₂O₄
• Číslo CAS: 41085-99-8
• Molekulová hmotnost: 933.87
• UNSPSC Code: 12171500
• NACRES: NA.32
• PubChem Substance ID: 57650110
• MDL number: MFCD00142354
• Beilstein/REAXYS Number: 8251870

Vlastnosti

• product line: BioReagent
• Quality Segment: 100
• assay: ≥98.0% (TLC)
• mol wt: 933.87 g/mol
• color: dark violet
• mp: 68 °C (dec.) (lit.)
• solubility: DMF: soluble, DMSO: soluble, methanol: soluble
• fluorescence: λ_ex 550 nm; λ_em 567 nm in phosphate buffer/SDS pH 7.0
• suitability: suitable for fluorescence
• SMILES string: [O-]Cl(=O)(=O)=O.CCCCCCCCCCCCCCCCCCN1c2ccccc2C(C)(C)/C1=C\C=C\C3=[N+](CCCCCCCCCCCCCCCCCC)c4ccccc4C3(C)C
• InChI: 1S/C59H97N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-41-50-60-54-46-39-37-44-52(54)58(3,4)56(60)48-43-49-57-59(5,6)53-45-38-40-47-55(53)61(57)51-42-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-40,43-49H,7-36,41-42,50-51H2,1-6H3;(H,2,3,4,5)/q+1;/p-1
• InChI key: JVXZRNYCRFIEGV-UHFFFAOYSA-M

Popis

General description

DiI is a Dye Aye, a chemical with CAS number 41085-99-8. It is a fluorescent lipophilic cationic indocarbocyanine dye, which is usually made as a perchlorate salt. Mostly used for scientific staining purposes, such as single molecule imaging, fate mapping and neuronal tracing (as DiI is retained in the lipid bilayers).

Application

1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate is used for the following applications:

• Preparation of Dil solution[1]
• fluorescent membrane dye[2][3][4]
• Di-I Staining[5]

Biochem/physiol Actions

Lipophilic carbocyanine dye used primarily for optical recordings of membrane voltage and for studies of membrane fluidity. Retrograde stain for neurons; provides intense, long-lasting staining of live neurons in vivo and in vitro.

Other Notes

Lipophilic fluorescent probe for studying membranes[6][7]

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)