PHR1396

Methotrexate

Pharmaceutical Secondary Standard; Certified Reference Material

• Synonyma: Methotrexate, (+)-Amethopterin, 4-Amino-10-methylfolic acid, 4-Amino-N¹⁰-methylpteroyl-L-glutamic acid, Antifolan, MTX, Methylaminopterin
• Empirický vzorec (Hillův zápis): C₂₀H₂₂N₈O₅
• Číslo CAS: 59-05-2
• Molekulová hmotnost: 454.44
• UNSPSC Code: 41116107
• NACRES: NA.24
• PubChem Substance ID: 329823370
• EC Number: 200-413-8
• Beilstein/REAXYS Number: 70669
• MDL number: MFCD00064370
• eCl@ss: 25140904

Vlastnosti

• Quality Segment: 300
• grade: certified reference material, pharmaceutical secondary standard
• agency: traceable to Ph. Eur. M1000000, traceable to USP 1414003
• API family: methotrexate
• CofA: current certificate can be downloaded
• technique(s): HPLC: suitable, gas chromatography (GC): suitable
• application(s): pharmaceutical; pharmaceutical (small molecule)
• format: neat
• storage temp.: 2-8°C
• SMILES string: CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
• InChI: 1S/C20H22N8O5/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27)/t13-/m0/s1
• InChI key: FBOZXECLQNJBKD-ZDUSSCGKSA-N
• Gene Information: human ... DHFR(1719)

Popis

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Methotrexate is an antagonist of folic acid that inhibits dihydrofolate reductase (DHFR) enzyme, reversibly and results in impaired DNA synthesis and cellular replication. Methotrexate has been used in treatment of rheumatoid arthritis and also for maintenance therapy in childhood acute lymphocytic leukemia (ALL).

Application

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Methotrexate may be used as a pharmaceutical secondary reference standard for the determination of the analyte in plasma samples and pharmaceutical formulations by various techniques.

Analysis Note

These secondary standards offer multi-traceability to the USP and EP (PhEur) primary standards, where they are available.

Other Notes

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

To see an example of a Certificate of Analysis for this material enter LRAB9958 in the slot below. This is an example certificate only and may not be the lot that you receive.

Bezpečnostní informace

• Piktogramy: GHS06,GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H301,H341,H360D,H372
• Bezpečnostní pokyny: P202 - P260 - P264 - P280 - P301 + P310 - P405
• Hazard Classifications: Acute Tox. 3 Oral - Muta. 2 - Repr. 1B - STOT RE 1
• Cílové orgány: Liver,Bone marrow
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Co se děje?: WGK 3
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable