10107921001

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

• Synonyma: s7 nuclease
• UNSPSC Code: 12352204
• NACRES: NA.54
• eCl@ss: 32160703
• Specific activity: ~15,000 U/mg
• Biological source: Staphylococcus aureus

Vlastnosti

• biological source: Staphylococcus aureus
• form: lyophilized
• specific activity: ~15,000 U/mg
• packaging: pkg of 15,000 U
• manufacturer/tradename: Roche
• optimum pH: 7.5
• shipped in: wet ice

Popis

Application

• Removal of endogenous RNA from in vitro translation systems
• RNA sequencing experiments

Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

Biochem/physiol Actions

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca²⁺ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

Preparation Note

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A₂₆₀.

Bezpečnostní informace

• Piktogramy: GHS07,GHS08
• Výstražné slovo: Danger
• Kódy nebezpečí: H315,H317,H319,H334,H335
• Bezpečnostní pokyny: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Cílové orgány: Respiratory system
• Skladovací třída: 11 - Combustible Solids
• WGK: WGK 1
• Bod vzplanutí (°F): does not flash
• Bod vzplanutí (°C): does not flash