Přejít k obsahu
Merck

SCMF002

FibroGRO®-LS Complete Media Kit for Culture of Human Fibroblasts

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

O této položce

UNSPSC Code:
12161503
NACRES:
NA.32
eCl@ss:
32161000

Hlavní dokumenty

Zobrazit celou dokumentaci
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohli
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohli

type

for cell culture

form

liquid

technique(s)

cell culture | mammalian: suitable

Quality Level

100

Application

FibroGRO-LS Medium is optimized for low-serum (2%) culture of human fibroblasts, and supports the growth of these cells in a 2% serum environment at rates equal to or greater than comparable media supplemented with much higher concentrations of FBS. May also be used for the culture of stromal cells, mesenchymal cells and mouse embryonic fibroblasts. FibroGRO-LS Medium contains no antimicrobials and no phenol red; components that can cause cell stress and “masking effects” that may influence experimental results (these are not needed, or recommended, to achieve optimal cell performance). FibroGRO provides a perfect cell system to establish low serum human feeder layers for stem cell culture, or as a model to study wound healing, toxicology or basic cell biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FibroGRO Media formulations provide an optimal cell culture environment for many types of human fibroblasts and mouse embryonic fibroblasts These low-serum/serum-free formulations have been shown to grow these cells at rates that meet or exceed commercially available serum containing media, while maintaining excellent cell morphology. The media are packaged in a specially designed UV protective shrinkwrap for added stability, which includes a temperature gauge for added convenience. FibroGRO Media contain no antimicrobials and no phenol red, components that can cause cell stress and masking effects that may influence experimental results.

Other Notes

SCMF-BM SCMF002-S

Preparation Note

SCMF-BM: Store at 4°C. See label for expiry date. SCMF001-S: Store at -20°C. See individual labels for expiry date. Supplemented medium = 4°C, in dark. Use within 1 month of supplementation. Do not freeze.

Legal Information

FIBROGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Warning

hcodes

H290

Hazard Classifications

Met. Corr. 1

Skladovací třída

8A - Combustible corrosive hazardous materials

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Související obsah

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

Stem Cell Research

This guide outlines our comprehensive selection of stem cells, media, supplements, growth factors, cultureware, and tools for stem cell characterization. These proven solutions cover a broad spectrum of stem cell and specialty cell culture areas and are backed by our knowledgeable technical support.

Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.

EpiGRO™, EndoGRO™ and FibroGRO™ Reagents

"The successful, reliable culture of epithelial cells is critical for many areas of research, including dermatology, respiratory research, and cancer research. Because the breakdown of control mechanisms in epithelial cells is a frequent contributor to cancer progression and metastasis, epithelial cell culture is particularly important for cancer research. EpiGRO™ media formulations are optimized to provide better viability, proliferation rates, morphology and culture stability than other commercially available options. The media are provided in unique, light-blocking, temperature-monitored packaging to ensure stability and protect the media from damage by light, contamination, and excessive heat. The media do not require or contain any antimicrobials or phenol red. These components can cause cell stress and influence experimental results by masking the true performance or health of the cell culture. Phenol red acts like an estrogen and may stimulate growth independently of experimental variables. "

Zobrazit všechny

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis