Přejít k obsahu
Merck

SCC165

BLaER1 Human B-cell Precursor Leukemia Cell Line

Human

Synonyma:

B cell Leukemia C/EBPalphaER clone 1

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

O této položce

UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32011203

Hlavní dokumenty

Zobrazit celou dokumentaci
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohli
Technický servis
Potřebujete pomoc? Náš tým zkušených odborníků je vám k dispozici.
Dovolte nám, abychom vám pomohli

Název produktu

BLaER1 Human B-cell Precursor Leukemia Cell Line, BLaER1 human B-cell precursor leukemia cell line may be transdifferentiated and used to model human monocytes.

biological source

human

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

Související kategorie

Application

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].

Biochem/physiol Actions

Cancer Cells

Analysis Note

  • Each vial contains ≥1×10 viable cells.
  • Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
  • Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
  • Cells are negative for mycoplasma contamination.
  • Cells tested positive for squirrel monkey retrovirus (SMRV).

General description

Induction of differentiation is a therapeutic approach for cancer. Treatment of leukemias with differentiation-inducing factors in combination with pro-apoptotic compounds has been shown to be highly effective . Ectopic expression of the transcription factor C/EBPa causes reprogramming of leukemia cells, resulting in expression of a normal cell phenotype.

The BLaER1 cell line is a tamoxifen-inducible derivative of the RCH-ACV B-cell leukemia cell line genetically modified using a retrovirus vector to express C/EBPa fused with the estrogen receptor hormone binding domain (ER) and GFP . A study demonstrated that BLaER1 cells could efficiently convert into cells exhibiting increased adherent, phagocytic and quiescent properties with a transcriptome resembling normal macrophages . Cells retained their phenotype even when C/EBPa was inactivated, a characteristic of genuine cell reprogramming. C/EBPa induction also impaired cell tumorigenicity after transplantation into immunodeficient mice. BLaER1 is thus a model for highly efficient transdifferentiation of B-cell-derived human cancer cells.

Source:
BLaER1 cells are a single subclone isolated from C/EBPaER-GFP-transfected RCH-ACV acute lymphoblastic leukemia (ALL) cells sorted for GFP expression. The parental cell line was obtained from the bone marrow of a female with chromosome translocation 1;19 and trisomy 8 .

Biosafety Level 2:
BLaER1 cells contain squirrel monkey retrovirus (SMRV) and should be handled under biosafety level 2.

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Bowen Zhou et al.
Science signaling, 15(765), eabl6781-eabl6781 (2022-12-21)
Pyroptosis is a mechanism of programmed, necrotic cell death mediated by gasdermins, a family of pore-forming proteins. Caspase-1 activates gasdermin D (GSDMD) under inflammatory conditions, whereas caspase-3 activates GSDME under apoptotic conditions, such as those induced by chemotherapy. These pathways
C/EBPα Induces Highly Efficient Macrophage Transdifferentiation of B Lymphoma and Leukemia Cell Lines and Impairs Their Tumorigenicity.
Francesca Rapino et al.
Cell reports, 19(6), 1281-1281 (2017-05-13)
S Waxman
Leukemia, 14(3), 491-496 (2000-03-17)
Successful treatment of acute promyelocytic leukemia (APL) has identified several novel approaches to induce leukemic cell differentiation and selective apoptosis by overcoming the site-specific transcriptional repression by dominant fusion leukemogenic proteins characteristic of APL and other forms of acute myelogenous
I Jack et al.
Cancer genetics and cytogenetics, 19(3-4), 261-269 (1986-01-15)
A cell line (RCH-ACV) was established from a bone marrow sample of a child with acute lymphoblastic leukemia (ALL). The cell line lacked Epstein-Barr virus nuclear antigen and exhibited a recently described nonrandom chromosome translocation, 1;19, thought to be associated
Francesca Rapino et al.
Cell reports, 3(4), 1153-1163 (2013-04-03)
Earlier work demonstrated that the transcription factor C/EBPα can convert immature and mature murine B lineage cells into functional macrophages. Testing >20 human lymphoma and leukemia B cell lines, we found that most can be transdifferentiated at least partially into
Zobrazit všechny související dokumenty

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis