PC522

Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb

liquid, Calbiochem^®

• Synonyma: Anti-ANT-1
• UNSPSC Code: 12352203

Vlastnosti

• Název produktu: Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11) Rabbit pAb, liquid, Calbiochem^®
• biological source: rabbit
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: dry ice
• storage temp.: −20°C

Popis

Disclaimer

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
doxorubicin-treated MCF7 cells, or breast carcinoma tissue

Application

Immunoblotting (2.5 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Paraffin Sections (1 g/ml, heat pre-treatment required)

General description

Anti-Adenine Nucleotide Translocase-1 (Ab-1) (2-11), rabbit polyclonal, recognizes ~35 kDa ANT-1 in doxorubicin-treated MCF-7 cells. It is validated for WB, immunofluorescence, and paraffin sections.

Purified rabbit polyclonal antibody. Recognizes the ~35 kDa ANT-1 protein.

Recognizes the ~35 kDa ANT-1 protein in doxorubicin-treated MCF-7 cells or breast carcinoma tissue.

• Antibody Target Gene Symbol: ANK3
• Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049
• Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)
• Hu Entrez ID: 288 (Related Antibodies: NB20, AP1034, PC523, AP1057)
• Mu Entrez ID: 11735
• Rat Entrez ID: 361833

Immunogen

Human

a synthetic peptide corresponding to amino acids 2-11 of human ANT-1

Other Notes

Bauer, M.K.A., et al. 1999. J. Cell Biol.147, 1493.
Marzo, I, et al. 1998. Science281, 2027.
Marzo, I, et al. 1998. J. Exp. Med.187, 1261.
Zoratti, M. and Szabo, I. 1995. Biochim. Biophys. Acta1231, 139.
Klingenberg, M. 1993. J. Bioenerg. Biomembr.25, 447.
Riccio, P., et al. 1975. FEBS Lett.56, 133.

MCF7 cells treated with doxorubicin at 0.2 µg/ml for 24 h can be used as positive control for immunoblotting. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany