OP64F

Anti-p21^WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

liquid, clone EA10, Calbiochem^®

• Synonyma: Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
• UNSPSC Code: 12352203

Vlastnosti

• Název produktu: Anti-p21^WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate, liquid, clone EA10, Calbiochem^®
• biological source: mouse
• antibody product type: primary antibodies
• clone: EA10, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• should not react with: mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• Quality Level: 100

Popis

Analysis Note

Negative Control
Unstimulated cells, unstimulated skin tissue, or SK-OV-3 cells

Positive Control
Any cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue

Application

Flow Cytometry (2 µg/ml)

Frozen Sections (5 µg/ml)

Immunoblotting (use Cat. No. OP64)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (use Cat. No. OP64)

Paraffin Sections (5 µg/ml, see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

This product has been discontinued.

Recognizes the ~21 kDa p21^WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).

Purified mouse monoclonal antibody generated by immunizing F1 mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~21 kDa p21^WAF1 protein.

Immunogen

Epitope: within amino acids 58-77 of human p21^WAF1

Human

full-length, recombinant, human p21^WAF1

Other Notes

Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology7, 889.
Deng, C., et al. 1995. Cell82, 675.
Waldman, T., et al. 1995. Cancer Res.55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff.5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res.54, 1169.
Li, R., et al. 1994. Nature371, 534.
Michieli, P., et al. 1994. Cancer Res.54, 3391.
Noda, A., et al.1994. Exp. Cell Res.211, 90.
El-Deiry, W.S., et al.1993. Cell75, 817.
Gu, Y., et al. 1993. Nature366, 707.
Harper, J.W., et al.1993. Cell75, 805.
Xiong, Y., et al.1993. Genes Devel.7, 1572.
Xiong, Y., et al.1993. Nature366, 701.
Xiong, Y., et al.1992. Cell71, 505.

Maximal p21^WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates p21^WAF1 expression. Serum stimulation of quiescent cells will give low level p21^WAF1 expression independent of p53 expression. Untreated cells will express little p21^WAF1 and can also be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. For staining paraffin sections, no pretreatment is usually needed, but some tissues may stain better after pretreating with heat or pressure cooker. In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21^WAF1 while the proliferating compartment of crypts will not stain. For immunoprecipitation and immunoblotting use Cat. No. OP64. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany