OP33
Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)
liquid, clone PAb1620, Calbiochem®
Synonyma:
Anti-p53 Antibody, Mouse Anti-p53, p53 Detection Antibody
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Doporučené produkty
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PAb1620, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat, primate, bovine
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
Recognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).
Immunogen
vLM mouse tumor cells
Application
Frozen Sections (see applications references)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/ml)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/ml, heat pre-treatment required)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/ml)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/ml, heat pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control
Hs27 cells or breast carcinoma tissue
Hs27 cells or breast carcinoma tissue
Other Notes
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Ball, R.K., et al. 1984. EMBO J.3, 1485.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Ball, R.K., et al. 1984. EMBO J.3, 1485.
Wild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
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