Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.
MRCF0R100
Microcon® DNA Fast Flow Centrifugal Filters
sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)
Synonyma:
Centrifuge concentrator
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Vybrat velikost
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
100 ea | K dispozici k odeslání DNESodAreál Kühne+Nagel spol. s r.o. | 15 900,00 Kč |
O této položce
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)
Working volume:
0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 100 ea
Technický servis
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Dovolte nám, abychom vám pomohliQuality Segment
material
Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade)
sterility
non-sterile
feature
holdup volume> 10 μL
packaging
pkg of 100 ea
manufacturer/tradename
Microcon®
parameter
0.5 mL sample volume
technique(s)
DNA purification: suitable, RNA purification: suitable
L
4.50 cm (1.8 in.)
diam.
12.3 mm
filtration area
0.32 cm2
working volume
0.5 mL
shipped in
ambient
General description
Microcon® centrifugal filters provide efficient concentration, desalting, or buffer exchange of aqueous biological samples ranging in volume from 10–500 μL. The Microcon® DNA fast flow filter is optimized for the concentration and recovery of genomic DNA with SDS buffer.
Application
Features and Benefits
Microcon® centrifugal filters have several benefits:
- high recovery for small volumes with reverse spin
- low-binding Ultracel® membrane
- fast processing
- yield concentration factors <20X for consistent reproducibility
Other Notes
Replaces: 42413
Legal Information
MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tato položka | |||
|---|---|---|---|
| description sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding) | description sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding), Ethylene Oxide (EtO) treated | description NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL | description NMWCO 10 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL |
| parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume | parameter 0.5 mL sample volume |
| manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® | manufacturer/tradename Microcon® |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| material Ultracel® regenerated cellulose membrane (low binding), polycarbonate device top, silicone O-ring (medical-grade), polyacetal base (membrane support), polypropylene tube (for filtrate/concentrate) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade, dual cycle ethylene oxide (EtO) treated) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) | material Ultracel® regenerated cellulose membrane (low binding), polyacetal base (membrane support), polycarbonate device top, polypropylene tube (for filtrate/concentrate), silicone O-ring (medical-grade) |
| working volume 0.5 mL | working volume 0.5 mL | working volume 0.5 mL | working volume 0.5 mL |
| packaging pkg of 100 ea | packaging pkg of 20 ea | packaging pkg of 100 ea | packaging pkg of 100 ea |
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Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?
1 answer-
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The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?
1 answer-
The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:
1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.
2.Sanitization Protocol:
A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).
B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.
C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).
D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.
E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.
F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.
G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.
DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.
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Has the color of the Microcon DNA Fast Flow become brighter blue?
1 answer-
Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.
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