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MABT520

Anti-Cytokeratin 18, clone 4D11E4 Antibody

Name: Anti-Cytokeratin 18, clone 4D11E4 Antibody
Brand: MM

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O této položce

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

4D11E4, monoclonal

Application:

IHC, WB

Citations:

Technický servis

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Vlastnosti

conjugate

unconjugated

Quality Segment

300

antibody form

ascites fluid

clone

4D11E4, monoclonal

purified by

(unpurified)

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG2b

UniProt accession no.

P05783

target post-translational modification

unmodified

Popis

General description

Purified recombinant fragment of human Cytokeratin 18 expressed in E. Coli.

~62 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Application

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected Cytokeratin 18 in human breast carcinoma, hepatocarcinoma, stomach cancer, and colon cancer tissues.

Physical form

Mouse monoclonal IgG2bascitic fluid containing 0.03% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting using cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected Cytokeratin 18 in HeLa, NIH/3T3, A549, Jurkat, MCF-7, HepG2, A431, and K562 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Skladovací třída

10 - Combustible liquids

wgk

WGK 1

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Související obsah

Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

White Paper: Further considerations of antibody validation and usage.

Globální číslo obchodní položky

Skladová položka	GTIN
MABT520	04055977171501