MABN2425

Anti-TRPV2 Antibody, clone 17A11

clone 17A11, from mouse

• Synonyma: Transient receptor potential cation channel subfamily V member 2, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, Vanilloid receptor-like protein 1, VRL-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 17A11, monoclonal
• species reactivity: mouse, rat
• packaging: antibody small pack of 25 μg
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_058903.2
• UniProt accession no.: Q9WUD2
• target post-translational modification: unmodified
• Gene Information: mouse ... Trpv2(22368) ; rat ... Trpv2(29465)

Popis

General description

Transient receptor potential cation channel subfamily V member 2 (UniProt: Q9WUD2; also known as TrpV2, Osm-9-like TRP channel 2, OTRPC2, Stretch-activated channel 2B, Vanilloid receptor-like protein 1, VRL-1) is encoded by the Trpv2 (also known as Sac2b, Vrl1) gene (Gene ID: 29465) in rat. TrpV2 is an ubiquitously expressed, calcium-permeable, non-selective cation channel with an outward rectification. It has been implicated in various physiological and pathological processes, including growth factor signaling, macrophage migration, cancer cell metastasis, heart disease, and neuronal cell development. It appears to be regulated by growth factors, like IGF1, PDGF, and morphogenetic neuropeptide/head activator. It is also activated by temperatures higher than 52 degree C, but is not activated by vanilloids and acidic pH. TrpV2 channel is upregulated by the nerve growth factor (NGF) via the mitogen-activated protein kinase (MAPK) signaling pathway to augment neurite outgrowth. Although NGF increases TrpV2 content, it does not induce the translocation of TrpV2 from cytoplasm to cell surface in PC12 cells. (Ref.: Cohen MR., et al. (2015). Mol. Cell Biol. 35(24): 4238-4252).

Specificity

Clone 17A11 detects Transient receptor potential cation channel subfamily V member 2 (TRPV2) in mouse and rat cells.

Immunogen

1D4-tagged full length rat Transient receptor potential cation channel subfamily V member 2 (TRPV2).

Application

Anti-TRPV2, clone 17A11, Cat. No. MABN2425, is a highly specific mouse monoclonal antibody that targets TRPV2 and has been tested for use in Immunocytochemistry and Western Blotting.

Western Blotting Analysis: A representative lot detected TRPV2 in Western Blotting applications (Cohen, M.R., et. al. (2013). PLoS One. 8(12):e85392).

Immunocytochemistry Analysis: A representative lot detected TRPV2 in Immunocytochemistry applications (Cohen, M.R., et. al. (2013). PLoS One. 8(12):e85392; Cohen M.R., et. al. (2015). Mol Cell Biol. 35(24):4238-52).

Quality

Evaluated by Immunocytochemistry in PC12 cells treated with NGF.

Immunocytochemistry Analysis: 2-10 µg/mL of this antibody detected TRPV2 in PC12 cells treated with mouse NGF.

Target description

86.71 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.