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Merck

MABC532

Anti-PTHrP Antibody, clone 4C4.1

clone 4C4.1, from mouse

Synonyma:

Parathyroid hormone-related protein, PTHrP, PTH-rP, Parathyroid hormone-like protein, PLP

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O této položce

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4C4.1, monoclonal
Application:
WB
Citations:
-
Technický servis
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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4C4.1, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PTHLH(5744)

General description

Parathyroid hormone-related protein (UniProt P12272; also known as Parathyroid hormone-like related protein, Parathyroid hormone-like hormone preproprotein, PLP, PTH-related protein, PTH-rP, PTHrP) is encoded by the PTHLH (also known as BDE2, HHM, PTHR, PTHRP) gene (Entrez Gene ID 5744) in human. Parathyroid hormone-related protein is initially translated as a 177 amino acid preproprotein with an N-terminal signal sequence (aa 1-24) and a propeptide sequence (aa 25-34), which are later removed to yield the mature protein. The mature protein is further cleaved to produce PTHrP[1-34] (aa 37-70), PTHrP[1-36] (aa 37-72), PTHrP[38-94] (aa 74-130), and PTHrP[107-139] (aa 143-175; also known as Osteostatin). PTHrP is produced in a wide variety of tissues, where it acts as a paracrine or autocrine factor to regulate cellular and organ growth, development, migration, differentiation, calcium transport, survival and apoptosis.
~16/6 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

GST-tagged recombinant human PTHrP lacking signal and propeptide sequence.

Application

Detect PTHLH using this mouse monoclonal Anti-PTHrP Antibody, clone 4C4.1, Cat. No. MABC532, validated for use in Western Blotting.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PTHrP in 10 µg of MCF-7 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.


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Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Související obsah

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.






Globální číslo obchodní položky

Skladová položkaGTIN
MABC53204055977177039