MAB4400

Anti-Renilla Luciferase Antibody, clone 5B11.2

ascites fluid, clone 5B11.2, Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 5B11.2, monoclonal
• Application: ELISA, Neutral, WB
• Citations: 34

Vlastnosti

• biological source: mouse
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 5B11.2, monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, neutralization: suitable, western blot: suitable
• isotype: IgG1
• UniProt accession no.: P27652
• shipped in: dry ice
• target post-translational modification: unmodified

Popis

General description

36 kDa

Renilla luciferase is a monomeric, bioluminescent protein derived from the sea pansy (Renilla reniformis). Renilla luciferase is often used as a reporter gene in sudies of gene expression or interaction. The 36 kDa enzyme catalyzes oxidation of the substrate coelenterazine to coelenteramide, resulting in the production of visible light. Renilla luciferase is not the same as luciferase from fireflies (Photalis pyralis) nor does it recognize the same substrates, so both enzymes can be used simultaneously in the same assay.

Immunogen

Renilla luciferase GST fusion protein

Application

Detect Renilla Luciferase using this Anti-Renilla Luciferase Antibody, clone 5B11.2 validated for use in ELISA, FUNC, NEUT & WB.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Western Blot:
A previous lot of this antibody was used in WB.

EIA:
A previous lot of this antibody was used in ELISA.

Neutralization:
A prevous lot of this antibody was used in NEAT.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Renilla luciferase

Physical form

Mouse monoclonal IgG1 Ascites.

Unpurified

Preparation Note

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Renilla

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable