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IPFL00010

Immobilon® -FL PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane with low background fluorescence

Immobilon®-FL PVDF Membrane

Synonyma:

Western blotting membrane, blotting membrane, transfer membrane

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Vám/Skladová položkaDostupnostCena
1 ea

Očekávané datum odeslání16. června 2026odAreál Kühne+Nagel spol. s r.o.

17 500,00 Kč

O této položce

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

17 500,00 Kč

Očekávané datum odeslání16. června 2026podrobné informace

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Název produktu

Immobilon®-FL PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Quality Segment

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 μm. It is designed for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL transfer membrane is hydrophobic and offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane offers optimal blotting for proteins with molecular weights greater than 20,000. The Immobilon®-FL membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection. Immobilon®-FL membrane can also be used for standard chemiluminescent or chromogenic detection.

Application

Immobilon-FL PVDF Membrane has been used in western blotting.[1][2]

Features and Benefits

Immobilon-FL Membrane:
  • The first transfer membrane optimized for fluorescence applications; Extremely low background enhances the sensitivity of all fluorescence detection protocols
  • Compatible with all commonly used fluorescent probes at a wide range of excitation/emission wavelengths
  • Best for multiplexing and chemifluorescence detections
  • compatible with standard blocking agents and buffers

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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IPFL20200IPFL10100IPFL00005
Immobilon&#174;-FL PVDF Membrane 1 roll, 27 cm x 3.75 m, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL00010

Immobilon®-FL PVDF Membrane

Immobilon&#174;-FL PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL20200

Immobilon®-FL PVDF Membrane

Immobilon&#174;-FL PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 &#181;m pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Millipore

IPFL10100

Immobilon®-FL PVDF Membrane

Millipore

Millipore

IPFL00005

Immobilon®-FL PVDF Membrane

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

detection method

chemiluminescent, fluorometric, colorimetric

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

detection method

chemiluminescent, colorimetric, fluorometric

compatibility

for use with All fluorescence dyes, for use with CPTS, for use with Ponceau-S red, for use with Amido black, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Coomassie brilliant blue

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

compatibility

for use with All fluorescence dyes, for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

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Skladovací třída

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

1–3 of 3 Questions  

  1. What is the thickness of this product?

    1 answer

    1. The thickness range of IPFL00010 is 110-145 microns.

      Helpful?

  2. Is 0.22 µm PVDF membrane available for fluorescent detection in Western blotting?

    1 answer

    1. Immobilon PSQ, with a pore size of 0.2 µm, is suitable for various detection methods including fluorescent, radioactive, chromogenic, and chemifluorescent detection. But when using Immobilon PSQ in Western Blotting, it is important to consider that there may be increased background noise, which might require more stringent wash steps to ensure accurate results.

      Helpful?

  3. Is it possible to use Immobilon FL instead of Immobilon P for western blots?

    1 answer

    1. Yes, Immobilon FL can be used as an alternative to Immobilon P for western blots.

      Helpful?

1 Review This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. Detroit, MI
    • Review 1
    • Votes 2
    5 out of 5 stars.

    Great for western blots

    I have been using this product for close to two and half years now. It is a highly reliable PVDF roll for western blotting. I use it to transfer a wide variety of proteins and use HRP conjugated secondary antibodies for imaging bands. The results have always been sharp, reliable and most importantly, reproducible. I shall buy again when we run out.

    Helpful?