GF16

Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2)

liquid, clone 236-5.2, Calbiochem^®

• Synonyma: Anti-Transforming Growth Factor-β3
• UNSPSC Code: 12352203

Vlastnosti

• Název produktu: Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2), liquid, clone 236-5.2, Calbiochem^®
• biological source: mouse
• antibody product type: primary antibodies
• clone: 236-5.2, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human, mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: human ... TGFB3(7043)

Popis

Analysis Note

Positive Control
Recombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells

Application

Frozen Sections (1-5 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.

Recognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.

This Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of TGF-β3 (Ab-1).

Immunogen

Epitope: within the N-terminal region

recombinant, human TGF-β3

Other Notes

Burton, P.B.J., et al. 1993 Int. J. Exp. Path.74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol]327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol.10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA85, 4715.
de Martin, R., et al. 1987 EMBO J.6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol.105, 1039.
Derynck, R., et al. 1985 Nature316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA77, 5258.

Does not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton^™ X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow