ABE1363

Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody

from rabbit, purified by affinity chromatography

• Synonyma: Histone H2A.Z, H2A/z
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: mouse, human
• Application: ChIP, DB, ELISA, ICC, WB
• Citations: 7

Vlastnosti

• biological source: rabbit
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• technique(s): ChIP: suitable (ChIP-seq), ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_002097
• UniProt accession no.: P0C0S5
• shipped in: wet ice
• target post-translational modification: acetylation (Lys5,Lys7,Lys11)
• Gene Information: human ... H2AFZ(3015)

Popis

General description

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

~16 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus.

Application

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.

This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11).

Biochem/physiol Actions

Human, mouse, wide range expected.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and preservative.

Analysis Note

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract.

Other Notes

Concentration: Please refer to lot specific datasheet.

Bezpečnostní informace

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable