AB6017
Anti-F-actin-capping protein subunit beta Antibody
from rabbit
Synonyma:
capping protein (actin filament) muscle Z-line, beta, F-actin capping protein beta subunit
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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Dovolte nám, abychom vám pomohliNázev produktu
Anti-F-actin-capping protein subunit beta Antibody, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
species reactivity (predicted by homology)
canine (based on 100% sequence homology), primate (based on 100% sequence homology), bovine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
bovine ... Capzb(338052)
dog ... Capzb(478209)
human ... CAPZB(832)
mouse ... Capzb(12345)
rat ... Capzb(298584)
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected F-actin-capping protein subunit beta in 10 µg of HeLa cell lysate.
Application
Detect F-actin-capping protein subunit beta using this Anti-F-actin-capping protein subunit beta Antibody validated for use in WB & IC.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect F-actin-capping protein subunit beta in NIH/3T3 and A431 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Biochem/physiol Actions
The antibody recognizes F-actin-capping protein subunit beta at the C-terminus of isoform 2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
F-actin-capping-protein subunit beta (CapZ beta) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. F-actin capping protein subunit beta is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of F-actin capping protein subunit beta’s subunits. While there is almost nothing known regarding the β3 subunit, the β1 and β2 isoforms each have distinct roles. The isoform β2 is predominantly expressed in non-muscle cells and is found in intercalated discs and the cell periphery, but is not observed at Z-discs. Isoform β1 is more greatly expressed in striated muscle and is localized at the Z-discs. It also contains the COOH-terminal extension necessary for capping the actin.
~ 34 kDa observed MW. There are 3 isoforms produced by alternative splicing: Isoform 1 at 31 kDa, Isoform 2 at 31 kDa, and Isoform 3 (sequence not available).
Immunogen
Epitope: C-terminus of Isoform 2
KLH-conjugated linear peptide corresponding to human F-actin-capping protein subunit beta of the C-terminus of isoform 2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Skladovací třída
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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