AB3242

Anti-PGC-1 Antibody

Chemicon^®, from rabbit

• Synonyma: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NM_008904
• UniProt accession no.: O70343
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... PPARGC1A(10891)

Popis

General description

PGC-1 is a transcription factor that determines whether preadipocytes will differentiate into brown or white fat cells. It interacts with PPARgamma to promote the expression of a cassette of genes which define the brown adipocyte phenotype.

Specificity

Immunogen peptide blocking prior to Western blotting completely abolished target band detection by this rabbit polyclonal antibody.

Immunogen

Epitope: C-terminus

Synthetic peptide from amino acids 777-797 (C-terminus) of mouse PGC-1.

Application

Anti-PGC-1 Antibody is a rabbit olyclponal antibody for the detection of PGC-1 also known as PPARgamma Coactivator 1-alpha & has been validated in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

ELISA Analysis: A 1:75,000 dilution of a previous lot was used in ELISA.

Immunocytochemistry Analysis: A 1:300 dilution of a previous lot was used in Immunocytochemistry.

Immunofluorescence Analysis: Representative lots detected PGC-1α in frozen mouse and rat muscle tissue sections (Gouspillou, G., et al. (2013). Longev. Healthspan. 2(1):13; Matsuura, T., et al. (2007). J. Orthop. Res. 25(11):1534-1540).

Immunohistochemistry Analysis: A representative lot detected PGC-1α in free-floating mouse forebrain sections (Tallaksen-Greene, S.J., et al. (2014). J Neurosci. 34(47):15658-15668).

Immunoprecipitation Analysis: Representative lots immunoprecipitated PGC-1α from mouse liver, skeletal muscle, and brown adipose tissue lysates (Boutant, M., et al. (2016). Cell Rep. 14(9):2068-2075; Park, R.D., et al. (2014). J. Exerc. Nutrition Biochem. 18(1):1-7; Kim, J.S., et al. (2014). J. Exerc. Nutrition Biochem. 18(3):259-266; Philip, A., et al. (2011). J. Biol. Chem. 286(35):30561-30570).

Western Blotting Analysis: Representative lots detected similar PGC-1α in human, mouse, and rat skeletal muscle tissue, as well as in nuclear and mitochondrial preparations from rat brown adipose tissue (Suwa, M., et al. (2015). J. Sports Sci. Med. 14(3):548-555; Lee, I., et al. (2015). Front. Pharmacol. 6:43; Vincent, G., et al. (2015). Front Physiol. 6:51; Lombardi, A., et al. (2015). PLoS One. 10(2):e0116498; Gouspillou, G., et al. (2014). FASEB J. 28(4):1621-1633; Saleem, A., et al. (2014). Am. J. Physiol. Cell Physiol. 306(3):C241-249).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blotting in mouse BAT extracts.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant mouse PGC-1, as well as endogenous PGC-1 in 15 µg of nuclear extract and whole cell lysate from mouse brown adipose tissue (BAT). Immunogen peptide blocking completely blocked target band detection.

Target description

~100 kDa observed. 91.03/89.64/91.71/77.14 kDa (human isoform 1/B4/B5/L-PGG-1alpha), 90.59 kDa (mouse isoform 1; PGC-1a1), 90.62 kDa (rat) calculated.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 20 mM PBS and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at -70ºC from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
BAT nuclear prep

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable