AB19015

Anti-MMP-2 Antibody, catalytic domain

Chemicon^®, from rabbit

• Synonyma: Gelatinase A, 72 kDa Type IV Collagenase
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 15

Vlastnosti

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NM_004530.2
• UniProt accession no.: P08253
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MMP2(4313)

Popis

Immunogen

E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain)

Epitope: catalytic domain

Application

Detect MMP-2 using this Anti-MMP-2 Antibody, catalytic domain validated for use in IP & WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Western blot: 1:2,000

Immunoprecipitation: 1:500

MMP-2 [Gelatinase-A] is constitutively produced in quiescent cells and tissues, and the enzyme has a high specific activity against denatured collagen. The low protein levels produced in cell culture (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, 1992) or immunoprecipitation. This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes rat MMP-2. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 72 kDa type IV collagenase (catalytic domain) as an immunogen.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in PBS, containing 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1048

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Skladovací třída: 12 - Non Combustible Liquids