565767

α-Secretase Substrate II, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).

• Synonyma: α-Secretase Substrate II, Fluorogenic, Ac-RE(EDANS)-VHHQKLVF-K(DABCYL)-R-OH
• Empirický vzorec (Hillův zápis): C₁₀₀H₁₄₄N₃₀O₂₀S
• Molekulová hmotnost: 2118.47
• NACRES: NA.77
• UNSPSC Code: 12352200

Vlastnosti

• Název produktu: α-Secretase Substrate II, Fluorogenic, An internally quenched fluorogenic peptide substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP).
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: red
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~340 nm; λ_em ~490 nm
• shipped in: ambient
• storage temp.: −20°C
• Quality Level: 100

Popis

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate containing the α-secretase cleavage site of β-Amyloid Precursor Protein (APP)

Product does not compete with ATP.

Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

An internally quenched fluorogenic peptide substrate that contains the α-secretase cleavage site of β-amyloid precursor protein (APP). Shown to be an effective substrate for yapsin-2, a glycosyl-phosphatidylinositol-linked aspartyl protease found in yeast (V_max/K_m = 0.031 min^-1). The cleavage has been shown to occur at the Lys-Leu bond. Excitation max: ~340 nm; emission max: ~490 nm.

Other Notes

Ac-Arg-Glu(EDANS)-Val-His-His-Gln-Lys-Leu-Val-Phe-Lys(DABCYL)-Arg-OH

Komano, H., et al. 1999. J. Biol. Chem. 274, 24431.
Komano, H., et al. 1998. J. Biol. Chem. 273, 31648.

Packaging

Packaged under inert gas

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable