539620

PP2A Inhibitor II, GST-Fusion, Human, Recombinant, E. coli - Calbiochem

• UNSPSC Code: 12352200

Vlastnosti

• assay: ≥90% (SDS-PAGE)
• form: liquid
• potency: 25-30 nM K_i
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C
• Quality Level: 100

Popis

Biochem/physiol Actions

Cell permeable: no

Primary Target
PP2A

Product does not compete with ATP.

Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

This product has been discontinued.

Recombinant, human PP2AI I₂ fused to GST and expressed in E. coli. Inhibits protein phosphatase 2A (PP2A) potently with K_i ~ 25-30 nM with myelin basic protein, histone H1, and other substrates of PP2A but not with casein as a substrate. Stimulates PP1. Prevents inhibition of E-Cdk-2 by p21^Cip1. Associates with B cyclin. Regulates histone binding to DNA, transcriptional activity and chromatin condensation. Undergoes phosphorylation in cells. Highly expressed in some cancers. Associates with HRX leukemia-associated fusion proteins. Occurs as fusion protein in acute undifferentiated leukemia. Does not inhibit PP1, PP2B, PP2C, or pyruvate dehydrogenase.

Other Notes

Li, M., et al. 1996. Biochemistry35, 6998.
Li, M., et al. 1995. Biochemistry34, 1988.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM Tris-HCl, 14 mM βME, 1 mM benzamidine, 1 mM EDTA, 0.1 mM PMSF, 10% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 10-13 - German Storage Class 10 to 13