235425

Caspase-3 Substrate II, Fluorogenic

Fluorogenic caspase-3 substrate.

• Synonyma: Caspase-3 Substrate II, Fluorogenic, Ac-DEVD-AMC
• Empirický vzorec (Hillův zápis): C₃₀H₃₇N₅O₁₃
• Číslo CAS: 169332-61-0
• Molekulová hmotnost: 675.64
• MDL number: MFCD00671411
• UNSPSC Code: 12352200
• NACRES: NA.77

Vlastnosti

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: DMSO: 10 mM
• fluorescence: λ_ex 365-380 nm; λ_em 430-460 nm
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(H,37,38)(H,39,40)(H,41,42)/t18-,19-,20-,26-/m0/s1
• InChI key: ALZSTTDFHZHSCA-RNVDEAKXSA-N

Popis

General description

Fluorogenic CPP32/Apopain substrate. Cleavage of this substrate by CPP32/Apopain shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Cleavage can be monitored by excitation at 380 nm and emission at 460 nm.

Fluorogenic caspase-3 substrate. Cleavage of this substrate by caspase-3 shows Michaelis-Menten kinetics (K_m = 9.7 ± 1.0 µM). Also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no

Km = 9.7 ± 1.0 µM for cleavage of this substrate by caspase-3

Primary Target
Fluorogenic caspase-3 substrate

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Glu-Val-Asp-AMC

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hermission, M., et al. 2000. Oncogene 19, 2338.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W., et al. 1995. Nature 376, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable