234155

Collagenase, Type II, Clostridium histolyticum

Collagenase, Type II, Clostridium histolyticum, is a crude collagenase preparation that contains higher clostripain and tryptic activity than Type I.

• Synonyma: Collagenase, Type II, Clostridium histolyticum
• Číslo CAS: 9001-12-1
• Číslo EC: 3.4.24.3 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• MDL number: MFCD00130830

Vlastnosti

• Quality Level: 100
• form: lyophilized
• specific activity: ≥200 collagenase units/mg dry wt
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• solubility: aqueous buffer: soluble
• foreign activity: Non-specific proteolytic ≥50 fluorescence units/mg
• shipped in: ambient
• storage temp.: 2-8°C

Popis

General description

Crude collagenase preparation that contains higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required.

Crude collagenase solid prepared to contain higher clostripain and tryptic activity than Type I. Useful in tissue dissociation studies in which a combination of collagenase and high proteolytic activity is required. Has an optimal pH of 7.0-8.0.

Preparation Note

Unstable in solution; reconstitute just prior to use.

Other Notes

Keller, S., and Mandl, I. 1963. Arch. Biochem. Biophys.101, 81.

One unit is defined as the amount of enzyme that will release 1.0 nmol Leu equivalent from collagen per min at 37°C, pH 7.2.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Bezpečnostní informace

• Skladovací třída: 10-13 - German Storage Class 10 to 13