218704

Casein Kinase II, α-Subunit, Human, Recombinant, E. coli

• Synonyma: rhCKIIα, Protein Kinase CKIIα
• UNSPSC Code: 12352202

Vlastnosti

• assay: ≥95% (SDS-PAGE)
• Quality Level: 100
• form: liquid
• specific activity: ≥1.0 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• shipped in: dry ice
• storage temp.: −70°C

Popis

General description

Note: 1 mU = 1 milliunit.

Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

Recombinant, human casein kinase II α-subunit expressed in E. coli. The catalytic subunit of serine/threonine protein kinase CKII. Suitable for use in reactions where the autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt sensitive with respect to its activity. Easily inactivated by treatment with heat, trypsin digestion, or urea.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 500 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 500 µM PMSF, 50% glycerol, pH 8.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Allende, J.E., and Allende, C.C. 1995. FASEB J. 9, 313.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Issinger, O.-G. 1993. Pharmacol. Ther. 59, 1.
Grankowski, N., et al. 1991. Eur. J. Biochem. 198, 25.

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 µmol phosphate from ATP to the synthetic casein kinase II substrate RRRDDDSDDD per min at 37°C, pH 8.5.

slight autoproteolysis yields one additional band of 39 kDa.however, no loss in activity is associated with the appearance of this 39 kDa product

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Bezpečnostní informace

• pictograms: GHS08,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H304,H411
• pcodes: P264 - P273 - P301 + P310 - P331 - P391 - P405
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Asp. Tox. 1
• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: 179.6 °F - closed cup
• flash_point_c: 82 °C - closed cup