208706

Ca²⁺/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

• Synonyma: CaM Kinase II
• UNSPSC Code: 12352202
• Specific activity: ≥250,000 units/mL
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in insect cells (S. frugiperda)

Vlastnosti

• recombinant: expressed in insect cells (S. frugiperda)
• Quality Level: 100
• assay: ≥90% (SDS-PAGE)
• form: liquid
• specific activity: ≥250,000 units/mL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• foreign activity: Phosphatases, proteases, none detected
• shipped in: wet ice
• storage temp.: −70°C

Popis

General description

Recombinant, rat Ca²⁺/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween^® 20 Detergent, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.

Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Standard Handling (A)

Bezpečnostní informace

• Skladovací třída: 10 - Combustible liquids
• wgk: WGK 1