14-476-M

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

• Synonyma: CDKN7, Cell division protein kinase 7, MO15, STK1, Serine/threonine-protein kinase 1
• UNSPSC Code: 12352202
• NACRES: NA.41
• eCl@ss: 32160405
• Form: liquid
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf21 cells
• Mol wt: M_w 37.8 kDa (cyclin H), M_w 39.9 kDa (cdk7), M_w 62.8 kDa (MAT1)

Vlastnosti

• biological source: human
• Quality Segment: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• form: liquid
• shelf life: 1 yr
• mol wt: M_w 37.8 kDa (cyclin H), M_w 39.9 kDa (cdk7), M_w 62.8 kDa (MAT1)
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• solubility: soluble
• NCBI accession no.: NM_001799.2
• UniProt accession no.: P50613
• storage temp.: −70°C
• Gene Information: human ... CDK7(1022)

Popis

General description

Research area: APOPTOSIS

Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC

Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells

Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.

Biochem/physiol Actions

The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.

Packaging

Also available in 250μg size, please inquire for pricing and availability.

Physical form

Ni²⁺/NTA-agarose

Preparation Note

1 year at -70°C

Analysis Note

routinely evaluated by phosphorylation of cdk7 substrate peptide

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Skladovací třída: 10 - Combustible liquids