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Merck

09-403

Anti-TKS5 (SH3 #1) Antibody

from rabbit

Synonyma:

Adaptor protein TKS5, Five SH3 domain-containing protein, SH3 and PX domains 2A, SH3 multiple domains 1, SH3 multiple domains protein 1, five SH3 domains

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O této položce

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Název produktu

Anti-TKS5 (SH3 #1) Antibody, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_008018.3

UniProt accession no.

O89032

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SH3PXD2A(9644)
mouse ... Sh3Pxd2A(14218)

Analysis Note

Control
Western Blot::
NIH-3T3 (100% confluent) lysate
Immunocytochemistry:
Mouse 3T3-Src(Y527F) cells
Routinely evaluated by Western Blot on NIH-3T3 (100% confluent) lysate.

Application

Anti-TKS5 (SH3 #1) Antibody detects level of TKS5 (SH3 #1) & has been published & validated for use in WB, IC & IP.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis: Antibody detected TKS5 in 20 μg of NIH-3T3 (100% confluent) lysate.

Biochem/physiol Actions

Other homologies: Human, Canine, Equine, Rat, and Bovine (100% sequence homology), Chicken (93% sequence homology)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

130-150 kDa Uncharacterized bands(s) may be observed in some cell lysates.
Tks5 (previously known as Fish), is a large scaffolding protein with one amino-terminal PX domain and five SH3 domains. It is cytoplasmic in normal fibroblasts. In Src-transformed cells, Tks5 localizes to podosomes. The coexpression of Tks5 and constitutively active Src in epithelial cancer cells resulted in the appearance of podosomes/invadopodia. Cells with reduced Tks5 expression are poorly invasive through matrigel. Tks5 is expressed and localized to podosomes/invadopodia in some invasive human cancer cell lines and tumor tissue, particularly breast cancers and melanomas. Thus, Tks5 appears to be required for podosome/invadopodia formation and for invasion of some cancer cells.

Immunogen

Epitope: 166-225 aa
Recombinant Protein, aa: 166-225. Homology: Human, Dog, Horse, Rat, Cow (100%, 60/60); Chicken (93%, 56/60)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

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Skladovací třída

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Karla C Williams et al.
Oncogene, 38(19), 3598-3615 (2019-01-18)
Invadopodia are cell protrusions that mediate cancer cell extravasation but the microenvironmental cues and signaling factors that induce invadopodia formation during extravasation remain unclear. Using intravital imaging and loss of function experiments, we determined invadopodia contain receptors involved in chemotaxis
Samuel J Ghilardi et al.
Molecular biology of the cell, 32(18), 1707-1723 (2021-07-01)
Interactions between the actin cytoskeleton and the plasma membrane are important in many eukaryotic cellular processes. During these processes, actin structures deform the cell membrane outward by applying forces parallel to the fiber's major axis (as in migration) or they
Nawal Bendris et al.
Journal of cell science, 129(14), 2804-2816 (2016-06-10)
The ability of cancer cells to degrade the extracellular matrix and invade interstitial tissues contributes to their metastatic potential. We recently showed that overexpression of sorting nexin 9 (SNX9) leads to increased cell invasion and metastasis in animal models, which
Deciphering the involvement of the Hippo pathway co-regulators, YAP/TAZ in invadopodia formation and matrix degradation.
Venghateri, et al.
Cell Death & Disease, 14, 290-290 (2023)
Sara K Donnelly et al.
The Journal of cell biology, 216(12), 4331-4349 (2017-10-25)
The initial step of metastasis is the local invasion of tumor cells into the surrounding tissue. Invadopodia are actin-based protrusions that mediate the matrix degradation necessary for invasion and metastasis of tumor cells. We demonstrate that Rac3 GTPase is critical
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