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07-850

Sigma-Aldrich

Anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody

Upstate®, from rabbit

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr507/pThr508)

Gene Information

human ... LIMK1(3984)

Specificity

Predicted to cross-react with Mouse, Rat, and Frog (100% homologous) based on sequence homology.
Recognizes phosphorylated LIMK1/2 (Tyr507/Thr508)

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat and chicken phospho-LIMK1/2 (Tyr507/Thr508)

Application

Detect phospho-LIMK 1/2 (Tyr507/Thr508) using this anti-phospho-LIMK 1/2 (Tyr507/Thr508) Antibody validated for use in WB.
Immunoblot Analysis: A 1:1000 dilution of this lot detected phosphorylated LIMK 1/2 (Tyr507/Thr508) in RIPA lysates of SDF-1α treated Jurkat cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Quality

Routinely evaluated by immunoblot.

Target description

72 kDa (LIMK1) and 65 kDa (LIMK2)

Physical form

100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
ImmunoAffinity Purified

Storage and Stability

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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