07-2184

Anti-acetyl-GAPDH (Lys160) Antibody

0.5 mg/mL, from rabbit

• Synonyma: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH
• eCl@ss: 32160702
• UNSPSC Code: 12352203
• NACRES: NA.41
• Clone: polyclonal
• Species reactivity: human
• Application: IP, WB, inhibition assay
• Citations: -

Vlastnosti

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): canine (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology), sheep (based on 100% sequence homology), feline (based on 100% sequence homology), porcine (based on 100% sequence homology), rat (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology), mouse (based on 100% sequence homology)
• concentration: 0.5 mg/mL
• technique(s): immunoprecipitation (IP): suitable, inhibition assay: suitable (peptide), western blot: suitable
• NCBI accession no.: NP_002037
• UniProt accession no.: P04406
• shipped in: wet ice
• target post-translational modification: acetylation (Lys160)
• Gene Information: human ... GAPDH(2597)

Popis

General description

Acetyl-GAPDH (Lys160), also known as Glyceraldehyde-3-phosphate dehydrogenase (GAPDH(lys160), or Peptidyl-cysteine S-nitrosylase GAPDH, and encoded by the gene GAPDH/GAPD, is an interesting multifunctional metabolic enzyme that plays a critical role in glycolysis but also many other important nuclear and cell functions such as transcription, RNA transport, DNA replication and even apoptosis and cell death. In glycolysis, GAPDH is a key enzyme that catalyzes the first step in the pathway by converting G3P into 3-phospho-D glyceroyl phosphate. An example of GAPDH’s regulation of gene transcription as a component of a GAIT complex, which selectively inhibits the activation of various gamma interferon induced inflammation responses. The protein-protein interactions of GAPDH are at least partially controlled by acetylation. For instance, transport of GAPDH into the nucleus is dependent upon acetylation at lysines 117, 227, and 251, and acetylation at lysine 183 governs co-factor interactions. Acetylation of GAPDH at lysine 160 ultimately is involved in p53 mediated cell death.

~37 kDa observed

Immunogen

Epitope: 0

KLH-conjugated linear peptide corresponding to the human GAPDH acetylated at Lys160.

Application

Peptide Inhibition Analysis: 1 µg/mL from a representative lot detected acetyl GAPDH (Lys60) in LPS/IFNgamma in RAW264.7 nuclear extract.

Immunoprecipitation Analyis: A representative lot immunoprecipitated acetyl GAPDH (Lys60) from LPS/IFNgamma treated RAW264.7 cell lysate (Prof. Akira Sawa, Johns Hopkins University, Baltimore.).

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

This Anti-acetyl-GAPDH (Lys160) antibody is validated for use in western blotting, peptide inhibition assay & IP for the detection of acetyl-GAPDH (Lys160).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in untreated and LPS/IFNgamma treated RAW264.7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected acetyl GAPDH (Lys60) in LPS/IFNgamma treated RAW264.7 cell lysate and not in untreated RAW264.7 cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable