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Key Documents

05-616-C

Sigma-Aldrich

Anti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free)

clone EE15, from mouse

Synonyma:

Nibrin, Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

EE15, monoclonal

species reactivity

human

should not react with

mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Nbn(27354)

General description

Nibrin (UniProt O60934; also known as Cell cycle regulatory protein p95, Nijmegen breakage syndrome protein 1, p95 protein of the MRE11/RAD50 complex) is encoded by the NBN (also known as AT-V1, AT-V2, ATV, NBS, NBS1, P95) gene (Gene ID 4683) in human. Nibrin is a component of the MRN (MRE11/RAD50/NBN) complex involved in early steps of DNA double strand breaks (DSBs). The phosphorylation of H2AX pSer139 (γ-H2AX) found at the DNA-damaged sites, for example, is mediated by a direct interaction between Nibrin and the phosphorylated mediator of the DNA damage checkpoint 1 (MDC1). ATM, CtIP/RBBP8, Tip60, BRCA1, and SMC1 are also known interaction partners of Nibrin. Nibrin contains a fork-head associated (FHA) domain and two BRCA1 C-terminal (BRCT) tandem domains in its N-terminal region, followed by two MRE11-binding motifs and an ATM-binding motif in its C-terminal region. The Ser278 and Ser343 residues are known to be targeted for phosphorylation by ATM in response to ionizing-radiation (IR). NBN gene mutations are responsible for Nijmegen breakage syndrome (NBS), a disorder characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer.

Specificity

Recognizes Nibrin/Nbs1, Mr 95kDa. A non-specific protein was also detected at Mr 33kDa in some lysates, but not Jurkat cell lysate.

Immunogen

Epitope: Amino acids 420-774.
GST fusion protein corresponding to residues 420-774 of human Nibrin/Nbs1.

Application

Anti-Nibrin/Nbs1 Antibody, clone EE15 (Ascites Free) is an antibody against Nibrin/Nbs1 for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected Nibrin/Nbs1 in A431 cells.
Western Blotting Analysis: A representative lot detected a shift of Nbs apparent molecular weight in lysates from gamma-irradiated human cells (U2OS, IMR90, HEK293, BJAB) (Wu, X., et al. (2000). Nature. 405(6785):477-482).
Immunocytochemistry Analysis: A representative lot detected NBS1-positive nuclear foci by fluorescent immunocytochemistry (Wu, X., et al. (2000). Nature. 289(5476):11).
Immunoprecipitation Analysis: 4 μg of this antibody immunoprecipitated Nibrin/Nbs1 from 500 μg of Jurkat RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Nibrin/Nbs1 in 10 µg of HeLa cell lysate.

Target description

~95 kDa observed.

Linkage

Replaces: 05-616

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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