05-368
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody
clone MPM-2, Upstate®, from mouse
Synonyma:
Phospho-Ser/Thr Antibody
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
MPM-2, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
phosphorylation (pSer/pThr)
General description
This antibody recognizes a variety of proteins that are phosphorylated during mitosis.
Upon entry into M (Mitosis) phase, many proteins are phosphorylated either directly or indirectly by M-phase-promoting factor (MPF). The MPM2 monoclonal antibody binds to a phospho amino acid-containing epitope (peptides containing LTPLK and FTPLQ domains) present on more than 50 proteins of M-phase eukaryotic cells.
Specificity
Recognizes phosphorylated serines or threonines when followed by a proline.
Immunogen
Mitotic human HeLa cell cytosolic lysate
Application
Anti-phospho-Ser/Thr-Pro MPM-2 Antibody is a Mouse Monoclonal Antibody for detection of phospho-Ser/Thr-Pro also known as Mitotic protein #2 & has been tested in ELISA, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Quality
routinely evaluated in immunoblot on colcemid treated human HeLa carcinoma cells
Target description
varies depending on the protein you are detecting
Physical form
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7% paraformaldehyde
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
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S A Innocente et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(5), 2147-2152 (1999-03-03)
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J Cobb et al.
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