05-118

Anti-FGF-2/basic FGF Antibody, clone bFM-2

clone bFM-2, Upstate^®, from mouse

• Synonyma: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: bFM-2, monoclonal
• Application: IHC, RIA, WB
• Citations: 86

Vlastnosti

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: bFM-2, monoclonal
• species reactivity: mouse, bovine, human, rat
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_002006.3
• UniProt accession no.: P09038
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FGF2(2247) ; mouse ... Fgf2(281161), Fgf2(14173) ; rat ... Fgf2(54250)

Popis

General description

17.5 kDa

Fibroblast growth factor 2 (UniProt: P03969; also known as FGF-2, Basic fibroblast growth factor (bFGF), Heparin-binding growth factor 2 (HBGF-2)) is encoded by the FGF2 gene (Gene ID: 281161) in bovine. Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm patterning in the early embryonic development. In adults, FGFs participate in several physiological and pathological processes, including cell proliferation, cell survival, differentiation, wound healing, and regulation of angiogenesis. FGF-2 is synthesized with a propeptide that is subsequently cleaved off to generate the mature active growth factor that can act as a monomer or a homodimer to act as a ligand for FGFR1, FGFR2, FGFR3, and FGFR4. Following its synthesis, it is exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF-2 occurs by direct translocation across the plasma membrane. It is shown that binding of exogenous FGF-2 to FGF receptors facilitates endocytosis followed by translocation of FGF-2 across endosomal membrane into the cytosol. Its import into nucleus requires the classical nuclear import machinery, involving Karyopherin a1 and b1 and the centrosomal protein 57 (CEP57). FGF-2 expression is observed in granulosa and cumulus cells. It is also expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. FGF-2 is also reported to act as an integrin ligand and binds to integrin aV:b3. This mouse monoclonal bFM-2 antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) is recommended for neutralizing activity. (Ref.: Matsuzaki, K., et al. (1989). Proc Natl Acad Sci USA. 86(24):9911-5; Campos, D.B., et al. (2010). Genet Mol Res. 9(1):309-23; Shimizu, T., et al. (2017). JCI Insight. 2(13):e93187; Zhao, H., et al. (2022). Pharm Biol. 60(1):334-346).

Immunogen

Purified bovine brain basic FGF (FGF-2, HBGF-2).

Application

Anti-FGF-2/basic FGF, clone bFM-2, Cat. No. 05-118, is a mouse monoclonal antibody that detects Basic fibroblast growth factor (bFGF) and is tested for use in Western Blotting and Immunohistochemistry.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human Basic fibroblast growth factor (bFGF) protein in Western Blotting application.

Immunohistochemistry Application: A representative lot detected FGF-2/basic FGF in Immunohistochemistry application (Zhao, H., et al. (2022). Pharm Biol. 60(1):334-346).

Western Blotting Analysis: A representative lot detected FGF-2/basic FGF in Western Blotting application (Shimizu, T., et al. (2017). JCI Insight. 2(13):e93187; Fukuoka, M., et al. (2018). PLoS One. 13(8):e0201796).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Biochem/physiol Actions

Clone bFM-2 is a mouse monoclonal antibody that specifically detects FGF-2/basic FGF. This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1) .

Important Note: This antibody can cross-react with native and heat-inactivated basic FGF, but will not neutralize biological activity (Uteza, Y., et al. (1999). Proc. Natl. Acad,. Sci. USA. 96(6); 3126-31).

Physical form

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis (WB): A 1:1000 dilution from a representative lot detected Basic fibroblast growth factor (bFGF) in Lysate from K562 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Skladovací třída: 12 - Non Combustible Liquids