05-118
Anti-FGF-2/basic FGF Antibody, clone bFM-2
clone bFM-2, Upstate®, from mouse
Synonyma:
Basic fibroblast growth factor, basic fibroblast growth factor bFGF, fibroblast growth factor 2, fibroblast growth factor 2 (basic), heparin-binding growth factor 2
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
bFM-2, monoclonal
species reactivity
mouse, bovine, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... FGF2(2247)
mouse ... Fgf2(281161) , Fgf2(14173)
rat ... Fgf2(54250)
Související kategorie
General description
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity. Upstate recommends anti-FGF-2/basic FGF, neutralizing (Catalog #05-117) for neutralizing activity.
Specificity
This antibody detects FGF-2/basic FGF.
This highly specific antibody does not cross-react with bovine acidic FGF (FGF-1, HBGF-1).
Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
Important Note: This antibody can cross-react with native and heat-inactivated basic FGF but will not neutralize biological activity (Uteza, Y., 1999). Upstate recommends anti-FGF-2/basic FGF, clone bFM-1 (neutralizing) (Catalog # 05-117) for neutralizing activity.
Immunogen
Purified bovine brain basic FGF (FGF-2, HBGF-2). Clone bFM-2.
Application
Detect FGF-2/basic FGF using this Anti-FGF-2/basic FGF Antibody, clone bFM-2 validated for use in IH, RIA & WB.
Immunohistochemistry:
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
Previous lots of this antibody have been shown to immunostain frozen brain sections fixed with ice-cold, ethanol:acetic acid [95:5]. This antibody has also been used at 10 μg/mL to stain paraffin-embedded sections (Matsuzaki, K., 1989).
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Quality
Evaluated on basic FGF (Catalog #01-114).
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected 100 ng FGF-2/basic FGF, carrier-free (Catalog # 01-114).
Target description
17.5 kDa
Physical form
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS, pH 7.4, purified by ammonium sulfate precipitation and DEAE Sephacryl chromatography. Frozen solution.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Fetal liver or kidney tissue.
Fetal liver or kidney tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ještě jste nenalezli správný produkt?
Vyzkoušejte náš produkt Nástroj pro výběr produktů.
recommended
Č. produktu
Popis
Stanovení ceny
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Immunohistochemical localization of cytokines in nevi.
M G Fleming, S F Howe, A G Candel
The American Journal of Dermatopathology null
Y Uteza et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 3126-3131 (1999-03-17)
We developed an experimental approach with genetically engineered and encapsulated mouse NIH 3T3 fibroblasts to delay the progressive degeneration of photoreceptor cells in dark-eyed Royal College of Surgeons rats. These xenogeneic fibroblasts can survive in 1. 5-mm-long microcapsules made of
Immunolocalization of acidic and basic fibroblast growth factors in porcine uterine and conceptus tissues
Gupta, A., et al
Biology of Reproduction, 56, 1527-1536 (1997)
Stefanie J Kirwin et al.
Investigative ophthalmology & visual science, 50(11), 5396-5404 (2009-05-29)
Inflammation is thought to play a role in disease progression and vision loss in diabetic retinopathy (DR). However, the level of inflammation and the role of cytokines and growth factors in the early stages of this disease are poorly understood.
Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model.
P L Faries, M L Marin, F J Veith, J A Ramirez, W D Suggs, R E Parsons, L A Sanchez, R T Lyon
Journal of Vascular Surgery null
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.